Journal article
Physicochemical and cytotoxicity analysis of glycerol monoolein-based nanoparticles
TE Hartnett, K Ladewig, AJ O'Connor, PG Hartley, KM McLean
Rsc Advances | ROYAL SOC CHEMISTRY | Published : 2015
DOI: 10.1039/c4ra13890b
Abstract
Lyotropic liquid crystalline dispersions, such as cubosomes, have been proposed as potential drug delivery vehicles. A recently described 'salt induced' method of cubosome production may be suitable for the encapsulation of macromolecular bioactive therapeutics, such as proteins, within the cubic phase. Here, we develop and characterise glycerol-monoolein (GMO)-based cubosomes using this novel method of cubosome production. Using the anionic biological lipid 1,2-dipalmitoyl phosphatidylserine (DPPS) to prevent GMO from forming its natural cubic-phase, we validate that addition of phosphate buffered saline (PBS) can be used to reverse the effects of DPPS. However, this transition is dependent..
View full abstractGrants
Funding Acknowledgements
T. E. Hartnett would like to thank The University of Melbourne, the Commonwealth Scientific and Industrial Research Organisation (CSIRO), and the Melbourne Materials Institute (MMI) for various scholarships. K. Ladewig acknowledges financial support from the Australian Research Council (ARC) in the form of an ARC Super Science Fellowship (FS 110200025). Infrastructure support from the Particulate Fluids Processing Centre (PFPC), the MMI, CSIRO and The University of Melbourne is gratefully acknowledged. This research was undertaken on the SAXS/WAXS beamline at the Australian Synchrotron, Victoria, Australia (M6531). Cryo-TEM images were obtained with the assistance of Ms Lynne Waddington, CSIRO, Parkville. Financial support was also provided by the National Health and Medical Research Council (NHMRC, Program Grant 1047603).